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Note:  Red text  indicates Holland Lab publications


Weakened APC/C activity at mitotic exit drives cancer vulnerability to KIF18A inhibition

Gliech, C.R., Yeow, Z.Y., Tapias-Gomez, D., Yang, Y., Huang, Z., Tijhuis, A.E., Spierings, D.CJ., Foijer, F., Chung, G., Tamayo, N., Bahrami-Nejad, Z., Collins, P., Nguyen, T.T., Plata Stapper, A., Hughes, P.E., Payton, M., and Holland, A.J. EMBO J. (2024).

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PLK4 self-phosphorylation drives the selection of a single site for procentriole assembly

Scott, P., Curinha, A., Gliech, C., and Holland, A.J. 

Journal of Cell Biology 222(12): e202301069. (2023).


Griseofulvin Radiosensitizes Non-Small Cell Lung Cancer Cells and Activates cGAS

Wang, X., Raman, N., Lemtiri-Chlieh, G., Chang, J., Jagtap, S., Chowdhury, D.D., Ballew, M., Carrieri, F.A., Nguyen, T., Nugent, K., Peck, T., Levine, M.S., Chan, A., Lam, C., Malek, R., Hoang, T., Phillips, R., Cheng, Z., Taparra, K., Connis, N., Hann, C.L., Holland, A., Tran, P. T., Lafargue, A., and Wang, H.

Molecular Cancer Therapeutics 22(4): 519-528. (2023).



PLK4 drives centriole amplification and apical surface area expansion in multiciliated cells

LoMastro, G.M., Drown, C.G., Maryniak, A.L., Jewett, C.E., Strong, M.A., and Holland, A.J.

eLife 11:e80643. (2022).


Centriole signaling restricts hepatocyte ploidy to maintain liver integrity

Sladky, V.C., Akbari, H., Tapias-Gomez, D., Evans, L., Drown, C., Strong, M., LoMastro, G., Larman, T., and Holland, A.J. Genes and Development 36:1-14. (2022).


Upstream open reading frames control PLK4 translation and centriole duplication in primordial germ cells

Phan, T.P., Boatwright, C.A., Drown, C.G., Skinner, M.W., Strong, M.A., Jordan, P.W. and Holland, A.J. Genes and Development 36(11-12): 718-736. (2022).

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Time is of the essence: the molecular mechanisms of primary microcephaly

Phan, T.P. and Holland, A.J. Genes and Development 35(23-24): 1551-1578. (2021).


TRIM37: a critical orchestrator of centrosome function.

Domínguez-Calvo, A., Gönczy, P., Holland, A.J., and Balestra, F.R. Cell Cycle 20(23): 2443-2451. (2021).


ANKRD26 recruits PIDD1 to centriolar distal appendages to activate the PIDDosome following centrosome amplification.

Evans, L.T., Anglen, T.*, Scott, P., Lukasik, K., Loncarek, J., and Holland, A.J. EMBO J 40(4). (2021).


Centrosome defects cause microcephaly by activating the 53BP1-USP28-TP53 mitotic surveillance pathway.

Phan, T., Maryniak, A.L.*, Boatwright, C.A., Lee, J., Atkina, A., Tijhuis, A., Spierings, D.C.J., Bazzi, H., Foijer, F., Jordan, P.W., Stracker, T.H., and Holland, A.J. EMBO J  40(1). (2021).


Clonal selection of stable aneuploidies in progenitor cells drives high-prevalence tumorigenesis.

Trakala, M., Aggarwal, M., Sniffen, C., Zasadil, L., Carroll, A., Ma, D., Su, X.A, Wangsa, D., Meyer, A., Sieben, C.J., Zhong, J., Hsu, P-H., Paradis, G., Ried, T., Holland, A., Van Deursen, J. and Amon, A

Genes and Development  35(15-16): 1079-1092. (2021).



Keeping track of time: The fundamentals of cellular clocks.

Gliech, C.R and Holland, A.J.* Journal of Cell Biology 291(11). (2020).


Targeting TRIM37-driven centrosome dysfunction in 17q23-amplified breast cancer.

Yeow, Z.Y.*, Lambrus, B.G.*, Marlow, R., Zhan, K.H., Durin, M., Evans, L.T., Scott, P.M., Phan, T., Park, E., Ruiz, L.A., Moralli, D., Knight, E.G., Badder, L.M., Novo, D., Haider, S., Green, C.M., Turr, A.N.J., Lord, C.J., Chapman, J.R.* and Holland, A.J.* Nature 585(7824). (2020).


Cell fitness screens reveal a conflict between LINE-1 retrotransposition and DNA replication.

Ardeljan, D., Steranka, J.P., Liu, C., Liz, C., Taylor, M.S., Payer, L.M., Gorbounov, M., Sarnecki, J.S., Deshpande, V., Hruban, R.H., Boeke, J.D., Fenyo, D., Wu, P.H., Smogorzewska, A., Holland, A. and Burns, K.H. Nature Structure and Molecular Biology 27(2): 168-178. (2020).


WBP11 is required for splicing the TUBGCP6 pre-mRNA to promote centriole duplication.

Park, E., Scott, P.M., Clutario, K.M., Cassidy, K.B., Zhan, K., Gerber and Holland, A.J. Journal of Cell Biology 219(1). (2019).



Massive centriole production can occur in the absence of deuterosomes in multiciliated cells.

Mercey, O.*, Levine, M.S.*, LoMastro, G., Rostaing, P., Brotslaw, E., Gomez, V., Kumar, A., Spassky, N., Mitchell, B.J., Meunier, A.* and Holland, A.J.* Nature Cell Biology 21: 1544-52. (2019). *equal contribution

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SFI1 promotes centriole duplication by recruiting USP9X to stabilize the microcephaly protein STIL.

Kodani, A., Moyer, T., Chen, A., Holland, A., Walsh, C.A. and Reiter, J.F. Journal of Cell Biology 218(7): 2185-87. (2019).


High-resolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy. 

Bowler, M., Kong, D., Sun, S., Nanjundappa, R., Evans, L., Farmer, V., Holland, A.J., Mahjoub, M.R., Sui, H. and Loncarek, J. Nature Communications 10(1): 993. (2019).


YAP and TAZ regulate cell volume.
Perez-Gonzalez, N.A., Rochman, N.D., Yao, K., Tao, J., Le, M.T., Flanary, S., Sablich, L., Toler, B., Crentsil, E., Takaesu, F., Lambrus, B., Huang, J., Fu, V., Chengappa, P., Jones, T.M., Holland, A.J., An, S., Wirtz, D., Petrie, R.J., Guan, K.L. and Sun, S.X. Journal of Cell Biology 218(10): 3472-88. (2019).


PLK4 promotes centriole duplication by phosphorylating STIL to link the procentriole to the microtubule wall.

Moyer, T.C. and Holland, A.J. eLIFE 8: e46054. (2019).

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The emerging link between centrosome aberrations and metastasis.

LoMastro, G.M. and Holland, A.J. Dev Cell  49(3): 325-31. (2019).


Mechanism and regulation of centriole and cilium biogenesis.

Breslow, D.K. and Holland, A.J. Ann Rev Biochem 88: 691-724. (2019).



Autoamplification and competition drive symmetry breaking: Initiation of centriole duplication by the PLK4-STIL Network.

Leda, M., Holland, A.J., and Goryachev, A.B. iScience 8: 222-35. (2018).


Global effects of DDX3 inhibition on cell cycle regulation identified by a combined phosphoproteomics and single cell tracking approach.

Heerma van Voss, M.R. Kammers, K., Vesuna, F., Brilliant, J., Bergman, Y., Tantravedi, S., Wu, X., Cole, R.N., Holland, A., van Diest, P.J. and Raman, V. Translational Oncology 11(3): 755-63. (2018).


Applying the auxin-inducible degradation system for rapid protein depletion in mammalian cells.

Lambrus, B.G., Moyer, T.C., and Holland, A.J. Methods in Cell Biol 358(6364): 716-8. (2017).


The impact of mitotic errors on cell proliferation and tumorigenesis.

Levine, M.S., and Holland, A.J. Genes and Development 32(9-10): 620-38. (2018).


Pushed out of a tough crowd: centrosome aberrations promote invasiveness.

Evans, L.T., and Holland, A.J. EMBO J 37(9): e99422. (2018).


Once and only once: mechanisms of centriole duplication and their deregulation in disease.

Nigg, E.A., Holland, A.J. Nature Reviews Molecular Cell Biology 19(5): 297-312. (2018).



Cell cycle proteins moonlight in multiciliogenesis.

Levine, M.S., and Holland, A.J. Science 358(6364): 716-8. (2017).


A new mode of mitotic surveillance.

Lambrus, B.G. and Holland, A.J. Trends in Cell Biology 8924(17): 30014-4. (2017).


Centrosome amplification is sufficient to promote spontaneous tumorigenesis in mammals.

Levine, M.S., Bakker, B., Boeckx, B., Moyett, J., Lu, J., Vitre, B., Spierings, D.C., Lansdorp, P.M., Cleveland, D.W., Lambrechts, D., Foijer, F. and Holland, A.J. Developmental Cell 40(3): 313-22. (2017).

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A USP28-53BP1-p53-p21 signaling axis arrests growth following centrosome loss or prolonged mitosis.

Lambrus, B.G., Daggubati, V., Uetake, Y., Scott, P.M., Clutario, K.M., Sluder, G., Holland, A.J. Journal of Cell Biology 214:143-153. (2016).

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CDK1 Prevents Unscheduled PLK4-STIL Complex Assembly in Centriole Biogenesis.

Zitouni, S., Francia, M.E., Leal, F., Montenegro Gouveia, S., Nabais, C., Duarte, P., Gilberto, S., Brito, D., Moyer, T., Kandels-Lewis, S., Ohta, M., Kitagawa, D., Holland, A.J., Karsenti, E., Lorca, T., Lince-Faria, M., Bettencourt-Dias, M. Current Biology 26(9):1127-37. (2016).



Chronic centrosome amplification without tumorigenesis.

Vitre, B.*, Holland, A.J.*, Kulukian, A.*, Shoshani, O., Hirai, M., Wang, Y., Maldonado, M., Cho, T., Boubaker, J., Swing, D.A., Tessarollo, L., Evans, S.M., Fuchs, E., and Cleveland, D.W. Proc. Natl. Acad. Sci. USA 112(46):E6321-30 (2015). *equal contribution.


Epidermal development, growth control, and homeostasis in the face of centrosome amplification.

Kulukian, A., Holland, A.J.*, Vitre, B.*, Naik, S., Cleveland, D.W., and Fuchs, E. Proc. Natl. Acad. Sci. USA 112(46):E6311-20 (2015). *equal contribution.


Generation of a conditional analog-sensitive kinase in human cells using CRISPR/Cas9-mediated genome engineering.

Moyer T.C., Holland A.J. Methods in Cell Biol 129:19-36. (2015).


p53 protects against genome instability following centriole duplication failure.

Lambrus, B.G., Uetake, Y., Clutario K.M., Daggubati, V., Snyder, M., Sluder, G., Holland, A.J. Journal of Cell Biology 210(1), 63-77. (2015).

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STIL binding to Plk4 activates kinase activity to promote centriole duplication.

Moyer T.C., Clutario K.M., Lambrus B.G., Daggubati V., Holland A.J. Journal of Cell Biology 209(6), 863-78. (2015).

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Preventing farnesylation of the dynein adaptor spindly contributes to the mitotic defects caused by farnesyltransferase inhibitors. 

Holland A.J., Reis R.M., Niessen S., Pereira C., Andres D.A., Spielmann H.P., Cleveland D.W., Desai A., Gassmann R. Molecular Biology of the Cell pii: mbc.E14-11-1560. (2015).



Polo-like kinase 4 inhibition: a strategy for cancer therapy?

Holland A.J., Cleveland D.W. Cancer Cell 26(2):151-3. (2014).


Polo-like kinase 4 shapes up.

Levine, M. S., Holland, A. J. Structure 22(8):1071-3 (2014).



Chromosome missegregation rate predicts whether aneuploidy will promote or suppress tumors.

Silk, A.D.*, Zasadil, L.M.*, Holland, A.J., Vitre, B., Cleveland, D.W., and Weaver, B.A. Proc. Natl. Acad. Sci. USA 44, E4134-4141. (2013). *equal contribution.


A two-step mechanism for epigenetic specification of centromere identity and function.

Fachinetti, D., Diego Folco, H., Nechemia-Arbely, Y., Valente, L.P., Nguyen, K., Wong, A.J., Zhu, Q., Holland, A.J., Desai, A., Jansen, L.E., Cleveland, D.W. Nature Cell Biology 15, 1056-66. (2013).


Catalytic assembly of the mitotic checkpoint inhibitor BubR1-Cdc20 by a Mad2-induced functional switch in Cdc20.

Han, J.S., Holland, A.J., Fachinetti, D., Kulukian, A., Cetin, B., Cleveland, D.W. Molecular Cell 51, 92-104. (2013).



The autoregulated instability of Polo-like kinase 4 limits centrosome duplication to once per cell cycle.

Holland, A.J., Fachinetti, D., Zhu, Q., Bauer, M., Verma, I.M., Nigg, E.A., and Cleveland, D.W. Genes and Development 26, 2684-2689. (2012).


An inducible system for the rapid and complete destruction of proteins in mammalian cells.

Holland, A.J.*, Fachinetti, D.* and Cleveland, D.W. Proc. Natl. Acad. Sci. USA 109, E3350-E3357. (2012). *equal contribution.


The deubiquitinase USP44 is a tumor suppressor that protects against chromosome missegregation.

Holland, A.J. and Cleveland, D.W. Journal of Clinical Investigation 122, 4325-4328. (2012).


Chromoanagenesis and cancer: mechanisms and consequences of localized, complex chromosomal rearrangements.

Holland, A.J. and Cleveland, D.W. Nature Medicine 18, 1630-1638. (2012).


Losing balance: the origin and impact of aneuploidy in cancer.

Holland, A.J. and Cleveland, D.W. EMBO Reports 13, 501-514. (2012).


Polo-like kinase 4 controls centriole duplication but does not directly regulate cytokinesis.

Holland, A.J., Fachinetti, D., Da Cruz, S., Zhu, Q., Vitre, B., Lince-Faria M., Verma, I.M., Bettencourt-Dias, M., and Cleveland, D.W. Molecular Biology of the Cell 23, 1838-1845. (2012).



Cep152 interacts with Plk4 and is required for centriole duplication.

Hatch, E.M., Kulukian, A., Holland, A.J., Cleveland, D.W., and Stearns, T. Journal of Cell Biology 191, 721-729. (2010).


Aurora kinases and protein phosphatase 1 mediate chromosome congression through regulation of CENP-E.

Kim, Y.*, Holland, A.J.*, Lan, W., and Cleveland, D.W. Cell 142, 444-455. (2010). *equal contribution.


Centriole duplication: A lesson in self-control.

Holland, A.J., Lan, W., and Cleveland, D.W. Cell Cycle 9, 2731-2736. (2010).


Cdc20 is required for the post-anaphase, KEN-dependent degradation of centromere protein F.

Gurden, M.D., Holland, A.J., van Zon, W., Tighe, A., Vergnolle, M.A., Andres, D.A., Spielmann, H.P., Malumbres, M., Wolthuis, R.M., Cleveland, D.W., and Taylor, S.S. Journal of Cell Science 123, 321-330. (2010).


Polo-like kinase 4 kinase activity limits centrosome overduplication by autoregulating its own stability.

Holland, A.J., Lan, W., Niessen, S., Hoover, H., and Cleveland, D.W. Journal of Cell Biology 188, 191-198. (2010).



Boveri revisited: chromosomal instability, aneuploidy and tumorigenesis.

Holland, A.J., and Cleveland, D.W. Nature Reviews Molecular Cell Biology 10, 478-487. (2009).


Requirements for NuMA in maintenance and establishment of mammalian spindle poles.

Silk, A.D., Holland, A.J., and Cleveland, D.W. Journal of Cell Biology 184, 677-690. (2009).



Beyond genetics: surprising determinants of cell fate in antitumor drugs.

Holland, A.J., and Cleveland, D.W. Cancer Cell 14, 103-105. (2008).


Many faces of separase regulation.

Holland, A.J., and Taylor, S.S. SEB Exp Biol Ser 59, 99-112. (2008).



Protein phosphatase 2A and separase form a complex regulated by separase autocleavage.

Holland, A.J., Bottger, F., Stemmann, O., and Taylor, S.S. Journal of Biological Chemistry 282, 24623-24632. (2007).



Cyclin-B1-mediated inhibition of excess separase is required for timely chromosome disjunction.

Holland, A.J., and Taylor, S.S. Journal of Cell Science 119, 3325-3336. (2006).



Disease-associated mutations cause premature oligomerization of myelin proteolipid protein in the endoplasmic reticulum.

Swanton, E., Holland, A., High, S., and Woodman, P. Proc Natl Acad Sci USA 102, 4342-4347. (2005).



The spindle checkpoint: a quality control mechanism which ensures accurate chromosome segregation.

Taylor, S.S., Scott, M.I., and Holland, A.J. Chromosome Research 12, 599-616. (2004).

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